Lysosome Biogenesis in C. elegans Coelomocytes: Shows lysosome
biogenesis in a coelomocyte in C. elegans. Endosomes are labeled
with RME 8::mRFP (red) and lysosomes with GFP::CUP-5 (green).
CUP-5 is the C. elegans orthologue of human TRPML1, mutations
in which results in the lysosomal storage disorder Mucolipidosis
Type IV. Note the biogenesis of a lysosome from an endosomal
compartment in lower left corner.
Mucolipidosis Type IV Lysosomal Defects: Shows an expanded
endosome/lysosome hybrid compartment in a cup-5 mutant coelomocyte
in C. elegans. CUP-5 is the C. elegans orthologue of human TRPML1,
mutations in which results in the lysosomal storage disorder
Mucolipidosis Type IV. The membrane of the expanded compartment
is marked by LMP-1::GFP (green) and contains endocytosed
This image shows an 8-cell stage of the mud snail Ilyanassa obsoleta embryo that was treated with a chemical antagonist of the NMDA receptor. This treatment disrupts the correct spindle orientation and pPKC localization, suggesting that NMDA receptor signaling is required for cell polarization during the early asymmetric cleavages of the mud snail. Nuclei (blue) are stained with DAPI, microtubules (green) and phosphorylated protein kinase C (pPKC) (red) are stained with their specific antibodies.
Nomarski image of Arabidopsis thaliana mature embryo
containing ProgericPro::GUS transgene, stained with X-Gluc,
fixed and cleared.
Whole mounts of WAP-TGF &alpha transgenic mouse mammary glands filled with pre-cancerous
hyperplasias overlaying the ductal epithelium of the gland. Inset in upper right corner
shows normal non-cancerous ductal epithelium
The Epidermal Growth Factor Receptor (green) residing on the membrane of breast cancer cells (cell nuclei in blue).
Immortalized mammary epithelial cells (MCF10A) growing as acini in a
basement membrane extracellular matrix. Images represent cellular
nuclei from the top (left) through to the middle of the acini.
Confocal images of an Arabidopsis heart stage embryo, surface view (left) and a medial cross-section (right).
These samples are from a wild-type plant with a transgene of the ATML1 promoter driving expression
of a nuclear localized YFP. Magnification approximately 40X.
One vascular bundle from a hand section of a wild-type Columbia Arabidopsis stem,
stained with Toluidine Blue and magnified 20X. From top to bottom, the photograph
includes pith (pink), xylem (blue), procambium (white.gray), phloem (pink), cortex
(brown/green) and epidermis (purple).
Immunofluorescence micrograph of a wild-type Chlamydomonas reinhardtii cell with
flagella and microtubule rootlets (green) stained with anti-acetylated alpha tubulin
and eyespot (red) stained with an antibody raised against an eyespot localized
protein, EYE3. Unpublished image taken by Joseph Boyd October 30, 2008.
Drosophila larval brain, immunostained for the stem cell and GMC marker, Worniu (red).
Cells in the optic lobe that, through genetic manipulations have become deficient for
Fragile X Protein, are labeled in green. DNA shown in blue.
The BMCB graduate program is an interdepartmental and interdisciplinary graduate program that seeks to equip students with the long-term knowledge and skills to succeed in careers in the life sciences. By training students to identify, ask and answer significant open questions in the biological sciences at the molecular level, we ultimately provide them with solid foundational knowledge in multiple areas of biology and help them to develop transferrable skills to adapt to the rapid pace of conceptual and technological advances in biology throughout their careers.
Our graduate program is funded by a training grant from the National Institute of General Medical Sciences (NIGMS)